PLANT CHROMOSOME ANALYSIS AND SORTING BY FLOW CYTOMETRY

被引：76
|
作者：
DOLEZEL, J
LUCRETTI, S
SCHUBERT, I
机构：
[1] ENEA,CRE CASACCIA,DEPT AGR RES & DEV,I-00100 ROME,ITALY
[2] INST PLANT GENET & CROP PLANT RES,DEPT CYTOGENET,D-06466 GATERSLEBEN,GERMANY
来源：
CRITICAL REVIEWS IN PLANT SCIENCES | 1994年 / 13卷 / 03期
关键词：
CHROMOSOME ISOLATION; FLOW KARYOTYPING; CHROMOSOME SORTING; FLOW CYTOGENETICS; GENE MAPPING; DNA LIBRARIES;
D O I：
10.1080/713608061
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
During the past decade, significant progress has been made in the development of methods for the preparation of plant chromosome suspensions suitable for flow cytometric analysis. In addition to successful classification of chromosomes (flow karyotyping), sorting of single chromosome types with a high degree of purity was reported in several plant species. Sorted chromosomes were used for the establishment of chromosome-specific DNA libraries and for gene mapping. The results confirmed the potential of plant flow cytogenetics and form a solid basis for further progress in this area. This article reviews its current status, analyzes major problems, and assesses future directions.
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收藏
页码：275 / 309
页数：35
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