FRAMESHIFTING IN THE EXPRESSION OF THE ESCHERICHIA-COLI TRPR GENE

被引：23

作者：

BENHAR, I ^{[1
]}

MILLER, C ^{[1
]}

ENGELBERGKULKA, H ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MOLEC BIOL,IL-91010 JERUSALEM,ISRAEL

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 19期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01457.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The trpR gene of Escherichia coli carries an open reading frame that encodes the trp repressor, 108 amino acids long. Here we show that translation of an additional (+1) reading frame of trpR occurs both in vivo and in vitro. This results in the synthesis of a stable +1 frame polypeptide. Using site-specific mutagenesis, immunological techniques and amino acid sequencing we have found that the N-terminus of the +1 frame product and that of the known 0 frame product are identical but that their C-termini differ. Our results are discussed in relation to the role of natural frameshifting as a regulatory mechanism of gene expression in general, and with respect to tryptophan biosynthesis in particular.

引用

页码：2777 / 2784

页数：8

共 35 条

[1] NORMAL TRANSFER-RNAS PROMOTE RIBOSOMAL FRAMESHIFTING [J].

ATKINS, JF ;

GESTELAND, RF ;

REID, BR ;

ANDERSON, CW .

CELL, 1979, 18 (04) :1119-1131

[2] RIBOSOME GYMNASTICS - DEGREE OF DIFFICULTY 9.5, STYLE 10.0 [J].

ATKINS, JF ;

WEISS, RB ;

GESTELAND, RF .

CELL, 1990, 62 (03) :413-423

[3] A PROCEDURE FOR AMINO-ACID SEQUENCING IN INTERNAL REGIONS OF PROTEINS [J].

BENHAR, I ;

ENGELBERGKULKA, H .

GENE, 1991, 103 (01) :79-82

[4] OVERLAPPING GENES IN RNA PHAGE - NEW PROTEIN IMPLICATED IN LYSIS [J].

BEREMAND, MN ;

BLUMENTHAL, T .

CELL, 1979, 18 (02) :257-266

[5] TRANSCRIPTION TERMINATION INVIVO IN LEADER REGION OF TRYPTOPHAN OPERON OF ESCHERICHIA-COLI [J].

BERTRAND, K ;

SQUIRES, C ;

YANOFSKY, C .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 103 (02) :319-337

[6] PROTECTION AGAINST FOOT-AND-MOUTH-DISEASE BY IMMUNIZATION WITH A CHEMICALLY SYNTHESIZED PEPTIDE PREDICTED FROM THE VIRAL NUCLEOTIDE-SEQUENCE [J].

BITTLE, JL ;

HOUGHTEN, RA ;

ALEXANDER, H ;

SHINNICK, TM ;

SUTCLIFFE, JG ;

LERNER, RA ;

ROWLANDS, DJ ;

BROWN, F .

NATURE, 1982, 298 (5869) :30-33

[7] PROGRAMMED RIBOSOMAL FRAMESHIFTING GENERATES THE ESCHERICHIA-COLI DNA POLYMERASE-III GAMMA SUBUNIT FROM WITHIN THE GAMMA-SUBUNIT READING FRAME [J].

BLINKOWA, AL ;

WALKER, JR .

NUCLEIC ACIDS RESEARCH, 1990, 18 (07) :1725-1729

[8] TRP REPRESSOR PROTEIN CONTROLS ITS OWN STRUCTURAL GENE [J].

BOGOSIAN, G ;

BERTRAND, K ;

SOMERVILLE, R .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 149 (04) :821-825

[9]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[10] INVITRO GENE FUSIONS THAT JOIN AN ENZYMATICALLY ACTIVE BETA-GALACTOSIDASE SEGMENT TO AMINO-TERMINAL FRAGMENTS OF EXOGENOUS PROTEINS - ESCHERICHIA-COLI PLASMID VECTORS FOR THE DETECTION AND CLONING OF TRANSLATIONAL INITIATION SIGNALS [J].

CASADABAN, MJ ;

CHOU, J ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1980, 143 (02) :971-980

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