IDENTIFICATION OF DEGRADATION ARTIFACTS FORMED UPON TREATMENT OF HYDROXYDIETHER LIPIDS FROM METHANOGENS WITH METHANOLIC HCL

被引：18

作者：

EKIEL, I

SPROTT, GD

机构：

[1] NATL RES COUNCIL CANADA,INST BIOL SCI,100 SUSSEX DR,OTTAWA K1A 0R6,ONTARIO,CANADA

[2] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1992年 / 38卷 / 08期

关键词：

HYDROXYDIETHER LIPIDS; DEGRADATION ARTIFACTS; METHANOLIC HCL; METHANOSARCINA-BARKERI;

D O I：

10.1139/m92-124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of purified 3-hydroxydiether lipid from Methanosarcina barkeri by standard conditions for head-group removal (2.5% methanolic HCl, 70-degrees-C) resulted in conversion to six identifiable degradation products. The four most abundant products were identified by mass spectrometry and nuclear magnetic resonance as monophytanylglycerol, 3-methoxydiether, and cis-trans isomers of a diether unsaturated between carbons 3 and 4. The latter lipid products may be mistaken for 2,3-di-O-phytanyl-sn-glycerol (standard diether) and 2-O-sesterterpanyl-3-O-phytanyl-sn-glycerol when separated by thin-layer chromatography.

引用

页码：764 / 768

页数：5

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