PHYTOANTIBODIES - A GENERAL VECTOR FOR THE EXPRESSION OF IMMUNOGLOBULIN DOMAINS IN TRANSGENIC PLANTS

被引：80

作者：

BENVENUTO, E

ORDAS, RJ

TAVAZZA, R

ANCORA, G

BIOCCA, S

CATTANEO, A

GALEFFI, P

机构：

[1] CNR, IST NEUROBIOL, I-00156 ROME, ITALY

[2] FAC BIOL JESUS ARIAS VELASCO, FISIOL VEGETAL LAB, E-33005 Oviedo, SPAIN

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 17卷 / 04期

关键词：

ISOLATED VH DOMAIN (IVD); SINGLE DOMAIN ANTIBODY (DAB); TRANSGENIC PLANTS;

D O I：

10.1007/BF00037067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1% of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development.

引用

页码：865 / 874

页数：10

共 50 条

[31] RECOMBINANT ANTIBODIES POSSESSING NOVEL EFFECTOR FUNCTIONS [J].