BIOSYNTHESIS OF PROSTAGLANDIN-D2, 15-KETOPROSTAGLANDIN-E2, AND HYDROXY FATTY-ACIDS BY RAM SEMINAL-VESICLE MICROSOMES

被引：8

作者：

RAVIKUMAR, PR ^{[1
]}

PAI, JK ^{[1
]}

ZMIJEWSKI, MJ ^{[1
]}

SIH, CJ ^{[1
]}

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 1979年 / 68卷 / 10期

基金：

美国国家卫生研究院;

关键词：

15‐Ketoprostaglandin E[!sub]2[!/sub]—biosynthesis; ram seminal vesicles; dependence on arachidonic acid; cofactors; Microsomes—seminal vesicles; prostaglandin synthesis; arachidonic acid metabolism; Prostaglandin D[!sub]2[!/sub]—biosynthesis; ram seminal vesicle microsomes; Seminal vesicles—microsomes;

D O I：

10.1002/jps.2600681028

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

At high arachidonic acid concentrations (164 μM) and without exogenous cofactors, ram seminal vesicle microsomes produced prostaglandin E2 and two less polar products, identified as prostaglandin D2 and 15‐ketoprostaglandin E2. The ratio of the biosynthetic products formed depended on the exogenous cofactor and on the arachidonic acid concentration. At high arachidonic acid concentrations (>150 μM), tryptophan, phenol, and glutathione stimulated prostaglandin E2 formation, but each affected the formation of the other prostaglandins differently. Ascorbic acid markedly stimulated hydroxy fatty acid formation. GLC‐mass spectral analysis of the hydroxy fatty acid fraction indicated the presence of 11‐hydroxy‐5,8,12,14‐eicosatetraenoic acid, 15‐hydroxy‐5,8,11,13‐eicosatetraenoic acid, and 12‐hydroxy‐5,8,10‐heptadecatrienoic acid. At low arachidonic acid concentrations (30 μM). glutathione still stimulated prostaglandin E2 biosynthesis, but the other cofactors stimulated 6‐ketoprostaglandin F1α” and hydroxy fatty acid formation. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company

引用

页码：1302 / 1306

页数：5

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