PROPIONYL-COA SYNTHETASE IN MAMMARY-GLAND AND LIVER OF COWS

被引:0
作者
GARRASTAZU, C [1 ]
GIMENEZ, MJ [1 ]
INIESTA, MP [1 ]
ARANGUEZ, MI [1 ]
RUIZAMIL, M [1 ]
机构
[1] UNIV COMPLUTENSE MADRID, FAC FARM, DEPT BIOQUIM & BIOL MOLEC, E-28040 MADRID, SPAIN
来源
REVISTA ESPANOLA DE FISIOLOGIA | 1991年 / 47卷 / 02期
关键词
GLUCONEOGENESIS; MAMMARY GLAND; PROPIONATE; RUMINANTS;
D O I
暂无
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Propionyl-CoA synthetase of liver and mammary gland from calf and midlactation cow was investigated. No activity of this enzyme was detected in calf mammary gland, but it was detected in calf liver. Propionyl-CoA synthetase was found in both, liver and mammary gland of the cow, although mammary gland activity was about 25% of that found in liver. The effects of pH and temperature on enzyme activity and stability were also investigated in crude extracts of liver and mammary gland tissues. The results suggest a different behaviour of the enzyme from both origins. Kinetic studies of the enzyme were also carried out, showing differences, depending on the organ, in the apparent substrate K(M) values.
引用
收藏
页码:63 / 68
页数:6
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