INVITRO MICRODIALYSIS - A NOVEL TECHNIQUE FOR STIMULATED NEUROTRANSMITTER RELEASE MEASUREMENTS

被引：18

作者：

BRADBERRY, CW ^{[1
]}

SPROUSE, JS ^{[1
]}

SHELDON, PW ^{[1
]}

AGHAJANIAN, GK ^{[1
]}

ROTH, RH ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT PSYCHIAT,NEW HAVEN,CT 06510

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1991年 / 36卷 / 01期

关键词：

SINGLE UNIT RECORDING; RELEASE; SEROTONIN; 3,4-METHYLENEDIOXYMETHAMPHETAMINE; DORSAL RAPHE; CORTEX; BRAIN SLICE; TRYPTOPHAN; MICRODIALYSIS;

D O I：

10.1016/0165-0270(91)90141-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel microdialysis technique suitable for parallel measurements of neurotransmitter release and single unit recordings from brain slices is presented. The effects of 3,4-methylenedioxymethamphetamine (MDMA) on slices of dorsal raphe nucleus and frontal cortex in a perfusion chamber for electrophysiological measures were studied. MDMA caused measurable release of serotonin which, in the case of the dorsal raphe, was of similar duration as the period of reduced cell firing induced by MDMA. Tryptophan potentiated the action of MDMA. Possible additional applications of this technique are discussed.

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页码：85 / 90

页数：6

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