CLONING, EXPRESSION AND FUNCTIONAL-ROLE OF XRPFI ALPHA-SUBUNITS AND BETA-SUBUNITS IN XENOPUS-LAEVIS OOCYTE

被引：5
作者：
GORGONI, B [1 ]
FIORENTINO, L [1 ]
MARCHIONI, M [1 ]
CARNEVALI, F [1 ]
机构：
[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOLEC,CNR,CTR STUDIO ACIDI NUCL,I-00185 ROME,ITALY
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 03期
关键词：
D O I：
10.1006/bbrc.1995.2575
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cDNAs coding for the alpha and beta components of XrpFI, an activator of ribosomal protein genes transcription, were isolated. RNA analysis showed that transcripts of the beta genes are present at constant level throughout Xenopus development unlike the beta proteins which are undetectable in the early stages of embryogenesis when transcription is silent. On the contrary, a transcripts and proteins were found in all the embryo stages examined. The role of the XrpFI a and beta subunits in rp-gene transcription has been studied by mRNA microinjection into X.laevis oocytes. The presence of the beta subunit appears to be critical for the proper rp-gene promoter function. (C) 1995 Academic Press, Inc.
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收藏
页码：1088 / 1095
页数：8
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