BDNF AND NT-3 INDUCE INTRACELLULAR CA2+ ELEVATION IN HIPPOCAMPAL-NEURONS

被引：182
作者：
BERNINGER, B
GARCIA, DE
INAGAKI, N
HAHNEL, C
LINDHOLM, D
机构：
[1] MAX PLANCK INST PSYCHIAT, DEPT NEUROCHEM & NEUROPHYSIOL, KLOPFERSPITZ 18A, D-82152 MARTINSRIED, GERMANY
[2] Univ Nacl Autonoma Mexico, FAC MED, DEPT FISIOL, MEXICO CITY 04510, DF, MEXICO
来源：
NEUROREPORT | 1993年 / 4卷 / 12期
关键词：
NEUROTROPHINS; FURA-2; TRKB; SYNAPTIC PLASTICITY;
D O I：
10.1097/00001756-199309150-00004
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
THE effects of neurotrophins on intracellular Ca2+ levels in rat hippocampal neurones were studied in vitro using fura-2 fluorescence microscopy. BDNF and NT-3, but not NGF, rapidly increased cytoplasmic Ca2+ concentrations in these neurones ten-fold to reach 1 muM. Moreover in some of the neurones both BDNF and NT-3 elicited Ca2+ responses, indicative of the presence of functional receptors for these neurotrophins in the same cell. In these cultures approximately 80% of the hippocampal neurones were stained with antibodies against full-length TrkB. The expression of functional TrkB was also confirmed by RNA analysis. These results demonstrate the presence of functional receptors for BDNF and NT-3 in hippocampal neurones.
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页码：1303 / 1306
页数：4
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