SINGLE TREE GENETIC-LINKAGE MAPPING IN CONIFERS USING HAPLOID DNA FROM MEGAGAMETOPHYTES

被引：142
作者：
TULSIERAM, LK
GLAUBITZ, JC
KISS, G
CARLSON, JE
机构：
[1] UNIV BRITISH COLUMBIA,BIOTECHNOL LAB,237 WESBROOK BLDG,6174 UNIV BLVD,VANCOUVER V6T 1W5,BC,CANADA
[2] BRITISH COLUMBIA FOREST SERV,KALAMALKA RES STN,VERNON V1B 2C7,BC,CANADA
来源：
BIO-TECHNOLOGY | 1992年 / 10卷 / 06期
关键词：
D O I：
10.1038/nbt0692-686
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.
引用
收藏
页码：686 / 690
页数：5
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