A COMMENT ON THE LOCALIZATION OF CYANINE DYE BINDING TO BRUSH-BORDER MEMBRANES BY THE FLUORESCENCE QUENCHING OF NORMAL-(9-ANTHROYLOXY) FATTY-ACID PROBES

被引：2
|
作者：
FARIA, JL
BERBERANSANTOS, M
PRIETO, MJE
机构：
[1] Centro de Química-Física Molecular, Instituto Superior Técnico, Lisboa
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1026卷 / 01期
关键词：
Cyanine dye; Energy transfer; Fluorescence quenching; n-(g-Anthroyloxy) fatty acid probe;
D O I：
10.1016/0005-2736(90)90343-M
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This work comments on the location and orientation of 3,3′-dipropylthiodicarbocyanine (diS-C3-(5)) in renal brush-border membrane vesicles (RBBMV) (Cabrini, G. and Verkman, A.S. (1986) Biochim. Biophys. Acta 862, 285-293) evaluated from collisional quenching of n-(9-anthroyloxy)stearic acid (n-AS) fluorescence. At variance with these authors, it is concluded that the quenching is due to resonance energy transfer. It is also shown that the fluorescence data are not clear evidence for the reported monomer and dimer locations. © 1990.
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页码：133 / 134
页数：2
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