THE EFFECT OF VESICAL OUTLET OBSTRUCTION ON THE PROTEIN SECONDARY STRUCTURE OF THE MUCOSA AND SEROSA IN RABBIT BLADDER WALL

被引：12
作者：
LIN, SY [1 ]
LIANG, RC [1 ]
YANG, CH [1 ]
HSU, HS [1 ]
LIN, ATL [1 ]
机构：
[1] VET GEN HOSP,DEPT SURG,DIV UROL,TAIPEI 11217,TAIWAN
来源：
UROLOGICAL RESEARCH | 1994年 / 22卷 / 04期
关键词：
BLADDER OUTLET OBSTRUCTION; MUCOSA; SEROSA; RABBIT; FT-IR; ATR; PROTEIN SECONDARY STRUCTURE;
D O I：
10.1007/BF00541893
中图分类号：
R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
1002 ; 100201 ;
摘要：
The biochemical composition of physiologically moist mucosa and serosa of rabbit bladder before and after bladder outlet obstruction was determined by means of FT-IR spectroscopy with the ATR method and second-derivative analysis. A predominantly beta-sheet structure was found in the amide I band for mucosa and serosa before and after obstruction, but the random coil structure increased in both obstructed bladder samples. However, the major beta-sheet structure associated with some alpha-helical structure in the amide II band of mucosa and serosa for non-obstructed bladder changed into a predominantly alpha-helical structure after bladder obstruction. The obstructed bladder serosa was more pronounced. The amount of glycoproteins doubled in the obstructed bladder serosa, but did not change in the bladder mucosa.
引用
收藏
页码：205 / 208
页数：4
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