ENZYME-ENTRAPPING MEMBRANES FOR BIOSENSORS OBTAINED BY RADIATION-INDUCED POLYMERIZATION

被引：26

作者：

DORETTI, L ^{[1
]}

FERRARA, D ^{[1
]}

LORA, S ^{[1
]}

机构：

[1] CNR,IST FOTOCHIM & RADIAZIONI ALTA ENERGIA,SEZ LEGNARO,I-35020 LEGNANO,ITALY

来源：

BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 9-10期

关键词：

BIOSENSOR; GLUCOSE SENSOR; ENZYME ELECTRODE; RADIATION-INDUCED POLYMERIZATION; ENZYME-ENTRAPPING MEMBRANE; GLUCOSE OXIDASE;

D O I：

10.1016/0956-5663(93)80029-O

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new method of physically immobilizing enzymes in poly(2-hydroxyethyl methacrylate) membranes was developed in order to obtain suitable biosensors. It was possible to prepare an enzyme sensor based on an oxygen Clark electrode and on glucose oxidase immobilized by low-temperature gamma radiation-induced polymerization. Temperature and pH effects on the activity of immobilized enzyme are described and the response characteristics of the resulting biosensor are summarized. The determination of glucose in standard solutions was carried out and a linear calibration curve, with an R(2) value of 0.9993, from the detection limit 5 x 10(-5) M to 1.2 x 10(-3) M was obtained. The biosensor was employed to analysis of control sera and the results were compared to those obtained by enzymatic-spectrophotometric detection.

引用

页码：443 / 450

页数：8

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