CHARACTERIZATION OF ESCHERICHIA-COLI STRAINS PRODUCING HEAT-STABLE ENTEROTOXIN-B (STB) ISOLATED FROM HUMANS WITH DIARRHEA

被引：62

作者：

LORTIE, LA ^{[1
]}

DUBREUIL, JD ^{[1
]}

HAREL, J ^{[1
]}

机构：

[1] UNIV MONTREAL,FAC VET MED,DEPT PATHOL & MICROBIOL,3200 SICOTTE,POB 5000,ST HYACINTHE J2S 7C6,QUEBEC,CANADA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 03期

关键词：

D O I：

10.1128/JCM.29.3.656-659.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two of 49 cytolethal distending toxin-producing strains of Escherichia coli isolated from human stools contained the gene coding for heat-stable enterotoxin b (STb), as detected by a colony hybridization assay. The STb gene was found to be on a 70-kb plasmid also coding for heat-labile enterotoxin (pLT-I). Restriction endonuclease analysis showed the STb gene from human isolates to be similar to the STb gene found in porcine strains. Moreover, by enzymatic amplification based on oligonucleotide primers designed from a porcine STb sequence, the expected portion of the STb gene was amplified for the human E. coli strains. The STb enterotoxin from these strains was bioactive in rat jejunal loops and was detected with an enzyme-linked immunosorbent assay by using polyclonal antiserum raised against purified porcine STb toxin.

引用

页码：656 / 659

页数：4

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