HUMAN OLIGODENDROCYTES ARE SUSCEPTIBLE TO CYTOLYSIS BY MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I-RESTRICTED LYMPHOCYTES

被引：41

作者：

RUIJS, TCG ^{[1
]}

FREEDMAN, MS ^{[1
]}

GRENIER, YG ^{[1
]}

OLIVIER, A ^{[1
]}

ANTEL, JP ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT NEUROL & NEUROSURG,MONTREAL H3A 2T5,QUEBEC,CANADA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1990年 / 27卷 / 2-3期

基金：

英国医学研究理事会;

关键词：

(Human); Cytotoxic lymphocyte; Multiple sclerosis; Oligodendrocyte;

D O I：

10.1016/0165-5728(90)90058-U

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The majority of human oligodendrocytes in enriched glial cell cultures expresses class I major histocompatibility complex (MHC) antigens. We used a 51Cr release assay to study the susceptibility of oligodendrocyte-enriched glial cells to MHC-restricted and non-restricted immune-mediated cytolysis. Mitogen-activated mononuclear cells induced significant lysis in a lectin-dependent cytotoxicity assay. Mononuclear cells allo-activated in a one-way mixed lymphocyte culture with E- cells from the glial cell donor induced a significantly higher degree of oligodendrocyte cytolysis than mononuclear cells activated with E- cells bearing MHC-class I antigens discordant with the glia. Cytolysis by alloactivated unfractionated lymphocytes and by purified CD8+ lymphocytes was reduced by an anti-class I antibody (W6/32). Our findings suggest that human oligodendrocytes can be susceptible targets for MHC class I-restricted lysis. © 1990.

引用

页码：89 / 97

页数：9