THE EFFECT OF HISTONE H1 AND DNA METHYLATION ON TRANSCRIPTION

被引：35

作者：

JOHNSON, CA ^{[1
]}

GODDARD, JP ^{[1
]}

ADAMS, RLP ^{[1
]}

机构：

[1] UNIV GLASGOW, INST BIOMED & LIFE SCI, DIV BIOCHEM & MOLEC BIOL, GLASGOW G12 8QQ, LANARK, SCOTLAND

来源：

BIOCHEMICAL JOURNAL | 1995年 / 305卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj3050791

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously shown that DNA methylation acts as a focus for the formation of inactive chromatin in vivo We have investigated the mechanism further by in vitro transcription of a template containing two tRNA genes and an extensive (G+C)-rich sequence characteristic of a CpG island. The extent of transcription from the unmethylated or fully methylated template was assayed in the presence of varied levels of histone H1. The transcriptional activity of both templates was inhibited by increasing amounts of histone H1, although inhibition with the methylated template occurs at a lower H1:DNA ratio. The H1c variant shows the greatest preferential inhibition of the methylated template. We demonstrate that histone H1 complexed to DNA is one of the factors that inhibits transcription by preventing the formation of initiation complexes, particularly on methylated template, rather than the formation of disordered H1 DNA aggregates.

引用

页码：791 / 798

页数：8

共 52 条

[1] DNA METHYLATION - THE EFFECT OF MINOR BASES ON DNA PROTEIN INTERACTIONS
ADAMS, RLP
[J]. BIOCHEMICAL JOURNAL, 1990, 265 (02) : 309 - 320
[2] SPECIFIC PROTECTION OF METHYLATED CPGS IN MAMMALIAN NUCLEI
ANTEQUERA, F
MACLEOD, D
BIRD, AP
[J]. CELL, 1989, 58 (03) : 509 - 517
[3] 5-METHYLCYTOSINE IS LOCALIZED IN NUCLEOSOMES THAT CONTAIN HISTONE H-1
BALL, DJ
GROSS, DS
GARRARD, WT
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (18): : 5490 - 5494
[4] DNA METHYLATION INHIBITS TRANSCRIPTION BY RNA POLYMERASE-III OF A TRANSFER RNA-GENE, BUT NOT OF A 5S RIBOSOMAL-RNA-GENE
BESSER, D
GOTZ, F
SCHULZEFORSTER, K
WAGNER, H
KROGER, H
SIMON, D
[J]. FEBS LETTERS, 1990, 269 (02) : 358 - 362
[5] THE ESSENTIALS OF DNA METHYLATION
BIRD, A
[J]. CELL, 1992, 70 (01) : 5 - 8
[6] BOURN D, 1994, IN PRESS DNA SEQUENC
[7] DNA METHYLATION INHIBITS TRANSCRIPTION INDIRECTLY VIA A METHYL-CPG BINDING-PROTEIN
BOYES, J
BIRD, A
[J]. CELL, 1991, 64 (06) : 1123 - 1134
[8] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9] THE TRANSCRIPTIONALLY-ACTIVE MMTV PROMOTER IS DEPLETED OF HISTONE H1
BRESNICK, EH
BUSTIN, M
MARSAUD, V
RICHARDFOY, H
HAGER, GL
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (02) : 273 - 278
[10] HISTONES AND DNA METHYLATION IN MAMMALIAN CHROMATIN - DIFFERENTIAL INHIBITION BY HISTONE-H1
CAIAFA, P
REALE, A
ALLEGRA, P
RISPOLI, M
DERME, M
STROM, R
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1090 (01) : 38 - 42

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