CHARACTERIZATION OF THE GENE FOR CHROMOSOMAL TRIMETHOPRIM-SENSITIVE DIHYDROFOLATE-REDUCTASE OF STAPHYLOCOCCUS-AUREUS ATCC 25923

被引：25
作者：
DALE, GE
THEN, RL
STUBER, D
机构：
[1] F HOFFMANN LA ROCHE & CO LTD,DEPT NEW TECHNOL & INFECT DIS,CH-4002 BASEL,SWITZERLAND
[2] F HOFFMANN LA ROCHE & CO LTD,DEPT PHARMACEUT RES,CH-4002 BASEL,SWITZERLAND
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1993年 / 37卷 / 07期
关键词：
D O I：
10.1128/AAC.37.7.1400
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The gene for the trimethoprim-sensitive (Tmp(s)) chromosomal dihydrofolate reductase (DHFR) of Staphylococcus aureus ATCC 25923 was cloned and characterized. The structural gene encodes a polypeptide of 159 amino acid residues and has a calculated molecular weight of 18,251. The amino acid sequences of this Tmp(s) DHFR and those of the trimethoprim-resistant type S1 DHFR encoded by transposon Tn4003 are 80% identical. In contrast to the trimethoprim-resistant enzyme, the Tmp(s) DHFR can be highly overexpressed in Escherichia coli, with most of the recombinant protein occurring in a soluble and an active form.
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页码：1400 / 1405
页数：6
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