JC VIRUS DETECTION BY INSITU HYBRIDIZATION IN BRAIN-TISSUE FROM ELDERLY PATIENTS

被引：59

作者：

MORI, M

KURATA, H

TAJIMA, M

SHIMADA, H

机构：

[1] TEIKYO UNIV,FAC MED,DEPT VIROL,TOKYO 173,JAPAN

[2] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL,DEPT PATHOL,TOKYO,JAPAN

来源：

ANNALS OF NEUROLOGY | 1991年 / 29卷 / 04期

关键词：

D O I：

10.1002/ana.410290414

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Brains from 10 patients aged 68 to 96 years at time of death were studied for JC viral DNA and common papovaviral capsid protein. In situ hybridization to JC viral DNA was performed by affinity cytochemistry using a biotinylated fragment of JC viral DNA. Immunohistochemistry was performed on brain tissue by the avidin DH-biotinylated horseradish peroxidase technique using polyclonal antibody raised against the papovaviral capsid protein. Viral protein and DNA were detected in 4 of 10 patients. JC virus may be present in the brains of aged patients more frequently than previously suspected.

引用

页码：428 / 432

页数：5

共 22 条

[1] PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY - INVESTIGATION OF 3 CASES USING INSITU HYBRIDIZATION WITH JC VIRUS BIOTINYLATED DNA PROBE [J].