EVALUATION OF COMBINED COMMERCIAL ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF ROTA AND ADENOVIRUSES FOR AUTOMATION

被引：2
|
作者：
CHEUNG, EY
HNATKO, SI
GUNNING, H
THOMAS, R
机构：
[1] Microbiology Division, Department of Laboratory Medicine, Royal Alexandra Hospital, Edmonton, Alta.
来源：
JOURNAL OF VIROLOGICAL METHODS | 1990年 / 30卷 / 03期
关键词：
AUTOMATION; COMMERCIAL ELISA KIT; ADENOVIRUS; ROTAVIRUS;
D O I：
10.1016/0166-0934(90)90076-R
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
219 stools were examined by direct electron microscopy (EM), culture and 'combined' commercial enzyme-linked immunosorbent assay kits (CELISA). The specificity of the combined ELISA for rotavirus was 100% as compared with EM, and 100% for adenovirus when both culture in addition to EM were carried out. ELISA appeared to be more sensitive than EM for both viruses. There was no cross-reaction between the 2 'combined' antisera. This technique may be useful for automation of viral diagnosis with ELISA using a 'panel' of selected viruses for a variety of specimens.
引用
收藏
页码：333 / 337
页数：5
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