AMPLIFICATION OF A LONG SEQUENCE THAT INCLUDES A PROCESSED PSEUDOGENE FOR ELONGATION FACTOR-II IN THE MOUSE

被引：9
作者：
KOIDE, T
ISHIURA, M
HAZUMI, N
SHIROISHI, T
OKADA, Y
UCHIDA, T
机构：
[1] NATL INST BASIC BIOL,DEPT CELL BIOL,DIV CELL FUS,OKAZAKI,AICHI 444,JAPAN
[2] OSAKA UNIV,INST MOLEC & CELLULAR BIOL,SUITA,OSAKA 565,JAPAN
[3] NATL INST GENET,MISHIMA,SHIZUOKA 411,JAPAN
来源：
GENOMICS | 1990年 / 6卷 / 01期
关键词：
D O I：
10.1016/0888-7543(90)90450-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Quantitative Southern blotting analysis has demonstrated that mouse cells contain about 70 copies per haploid genome of a DNA sequence related to the gene for elongation factor 2. The restriction maps of seven cosmids that each carry one copy of the EF2-related sequence (MER) and nucleotide sequences of MERs were highly conserved among the cosmids. Data obtained by such analyses suggest that MERs were produced by the integration of one copy of MER derived from poly(A)+ mRNA for EF2 into a specific site in the mouse genome, with subsequent amplification of MER together with its large flanking sequences during the evolution of the mouse. Furthermore, it appears that the size of each repeating unit is more than 60 kb. Analysis by pulse-field gel electrophoresis suggested that multiple copies of a repeating unit of more than 400 kb (or two units) are clustered at a specific site (or each specific site) in the mouse genome. © 1990.
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页码：80 / 88
页数：9
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