Single Nucleotide Polymorphisms Detection In PRM1 Gene Of Infertile Men

At least 50% of married couples are infertility; it is the major concern among them. The male partner is the sole contributor to the problem. These men are diagnosed as unexplained cases and with no a etiology or idiopathic cases. The current study was concerted to estimate the role of single nucleotide polymorphisms (SNPs) in the protamine-1 gene PRM1 of 160 human male (130 infertile and 30 fertile individuals) by Restriction Fragment Length Polymorphism RFLP assay. We were detected 95 sample with alleles carrying Guanine (G) in sequence 197G of PRM1 gene fragment, fertile control 16 (53.3%) and an infertile patient79 (69.7 %), and 38 patients: fertile control 8 (26.6%) and infertile 30 (23 %) carrying a G SNP, while a heterozygotes polymorphism G/T197 SNP variant were present in 27 sample: control 6(20%) and infertile 21 (16.1%), suggesting that there are G197T SNP of PRM1 in the studied population Thus, the RFLP assay may be provides a rapid and a simple screening method to identification the polymorphism.