STABLE EXPRESSION AND REGULATION OF A RAT-BRAIN K+ CHANNEL

被引：61
作者：
CRITZ, SD
WIBLE, BA
LOPEZ, HS
BROWN, AM
机构：
[1] Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas
来源：
JOURNAL OF NEUROCHEMISTRY | 1993年 / 60卷 / 03期
关键词：
NGK2; CHANNELS; PERMEATION; TRANSFECTION; HEK; 293; CELLS; KV3.1;
D O I：
10.1111/j.1471-4159.1993.tb03273.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Shaw-type K+ channel K(v)3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to K(v)3.1 channels expressed transiently in Xenopus oocytes. K(v)3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased K(v)3.1 currents. Based on these results, we find that the stable expression of voltage-gated K+ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.
引用
收藏
页码：1175 / 1178
页数：4
相关论文
共 19 条
[1]   A RAT-BRAIN NA+ CHANNEL ALPHA-SUBUNIT WITH NOVEL GATING PROPERTIES [J].
AULD, VJ ;
GOLDIN, AL ;
KRAFTE, DS ;
MARSHALL, J ;
DUNN, JM ;
CATTERALL, WA ;
LESTER, HA ;
DAVIDSON, N ;
DUNN, RJ .
NEURON, 1988, 1 (06) :449-461
[2]   AN AMINO-ACID MUTATION IN A POTASSIUM CHANNEL THAT PREVENTS INHIBITION BY PROTEIN-KINASE-C [J].
BUSCH, AE ;
VARNUM, MD ;
NORTH, RA ;
ADELMAN, JP .
SCIENCE, 1992, 255 (5052) :1705-1707
[3]  
Chabala L. D., 1992, Society for Neuroscience Abstracts, V18, P922
[4]  
CHANDY K G, 1992, Biophysical Journal, V61, pA257
[5]  
DREWE JA, 1992, J NEUROSCI, V12, P538
[6]   PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE BETA-1-SUBUNIT OF THE RAT-BRAIN SODIUM-CHANNEL [J].
ISOM, LL ;
DEJONGH, KS ;
PATTON, DE ;
REBER, BFX ;
OFFORD, J ;
CHARBONNEAU, H ;
WALSH, K ;
GOLDIN, AL ;
CATTERALL, WA .
SCIENCE, 1992, 256 (5058) :839-842
[7]   STRUCTURAL ELEMENTS INVOLVED IN SPECIFIC K+ CHANNEL FUNCTIONS [J].
JAN, LY ;
JAN, YN .
ANNUAL REVIEW OF PHYSIOLOGY, 1992, 54 :535-555
[8]   INACTIVATION OF CLONED NA CHANNELS EXPRESSED IN XENOPUS OOCYTES [J].
KRAFTE, DS ;
GOLDIN, AL ;
AULD, VJ ;
DUNN, RJ ;
DAVIDSON, N ;
LESTER, HA .
JOURNAL OF GENERAL PHYSIOLOGY, 1990, 96 (04) :689-706
[9]   SPECIFICATION OF SUBUNIT ASSEMBLY BY THE HYDROPHILIC AMINO-TERMINAL DOMAIN OF THE SHAKER POTASSIUM CHANNEL [J].
LI, M ;
JAN, YN ;
JAN, LY .
SCIENCE, 1992, 257 (5074) :1225-1230
[10]   ALTERNATIVE SPLICING CONTRIBUTES TO K+ CHANNEL DIVERSITY IN THE MAMMALIAN CENTRAL-NERVOUS-SYSTEM [J].
LUNEAU, CJ ;
WILLIAMS, JB ;
MARSHALL, J ;
LEVITAN, ES ;
OLIVA, C ;
SMITH, JS ;
ANTANAVAGE, J ;
FOLANDER, K ;
STEIN, RB ;
SWANSON, R ;
KACZMAREK, LK ;
BUHROW, SA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3932-3936
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