EXPRESSION OF A NOVEL TRANSCRIPT OF THE MYELIN BASIC-PROTEIN GENE

被引：43

作者：

KITAMURA, K ^{[1
]}

NEWMAN, SL ^{[1
]}

CAMPAGNONI, CW ^{[1
]}

VERDI, JM ^{[1
]}

MOHANDAS, T ^{[1
]}

HANDLEY, VW ^{[1
]}

CAMPAGNONI, AT ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,MENTAL RETARDAT RES CTR,760 WESTWOOD PLAZA,LOS ANGELES,CA 90024

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 06期

关键词：

Brain specific gene; cDNA; Gene expression; Messenger RNA; Myelin basic protein gene; Myelin protein;

D O I：

10.1111/j.1471-4159.1990.tb04908.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: A cDNA (M41) corresponding to a mouse myelin basic protein (MBP) mRNA with a longer 5′‐untranslated region than predicted from earlier studies of MBP gene structure has been isolated and characterized. The additional 5′‐untranslated region is encoded by two previously unidentified exons upstream of the major transcription start site of the gene. Using a DNA probe specific for M41‐MBP mRNAs, Northern blot analysis indicated that expression of this transcript follows a developmental course in mouse brain similar to that of the majority of MBP mRNAs, but that the level of expression varies between brain and spinal cord. Expression of MBP mRNAs similar to the mouse M41‐MBP also was identified in rat brain. The results suggest that the structure of the MBP gene is more complex than originally thought, containing at least two more exons. There appears to be at least one more MBP gene promoter that directs the synthesis of a subset of MBP mRNAs with a unique 5′‐untranslated region. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：2032 / 2041

页数：10

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