CHEMILUMINESCENCE ASSOCIATED WITH DOXORUBICIN-INDUCED LIPID-PEROXIDATION IN RAT-HEART MITOCHONDRIA

被引：0

作者：

KITADA, M ^{[1
]}

HORIE, T ^{[1
]}

AWAZU, S ^{[1
]}

机构：

[1] TOKYO COLL PHARM,DEPT BIOPHARMACEUT,HACHIOJI,TOKYO 19203,JAPAN

来源：

BIOCHEMICAL PHARMACOLOGY | 1994年 / 48卷 / 01期

关键词：

CHEMILUMINESCENCE; DOXORUBICIN; HEART MITOCHONDRIA; OXIDATIVE STRESS; LIPID PEROXIDATION; REACTIVE OXYGEN SPECIES; SINGLET OXYGENS; EXCITED CARBONYLS;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Chemiluminescence was observed in a rat heart mitochondrial suspension containing NADH, FeCl3 and doxorubicin (Adriamycin(R)) (DXR). There was good correlation between the total intensity of chemiluminescence and the total amount of thiobarbituric acid reactive substances (TBARS) produced during DXR redox cycling. Thus, the chemiluminescence was shown to be associated with lipid peroxidation. The chemiluminescence was quenched by superoxide dismutase (SOD), suggesting that superoxide anion radicals contributed to its production. Upon addition of 1,4-diazabicyclo[2,2,2]octane (DABCO), a singlet oxygen emission enhancer, to the mitochondrial suspension emitting the chemiluminescence, the chemiluminescence intensity increased transiently, indicating the involvement of singlet oxygen. Furthermore, spectral analysis of the chemiluminescence showed it to be due to singlet oxygen and excited carbonyls.

引用

页码：93 / 99

页数：7

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