FURTHER CONCERNS OVER CHENG-PRUSOFF ANALYSIS

被引：168

作者：

LEFF, P

DOUGALL, IG

机构：

[1] Fisons Research and Development Laboratories, Loughborough, Leics. LE11 ORH, Bakewell Road

[2] Department of Pharmacology, Fisons Research and Development Laboratories, Loughborough, Leics. LE11 ORH, Bakewell Road

来源：

TRENDS IN PHARMACOLOGICAL SCIENCES | 1993年 / 14卷 / 04期

关键词：

D O I：

10.1016/0165-6147(93)90080-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In his article in last month's issue of TiPS (14, 89-91), Douglas Craig discussed the application of the Cheng-Prusoff relationship to the analysis of antagonists in functional experiments. Craig described how the translation of this method from biochemistry to pharmacology can be accompanied by errors due to theoretical misunderstanding. He also emphasized the lower level of rigour associated with this means of estimating antagonist affinity constants compared with Schild analysis. In this article, Paul Leff and Iain Dougall extend Craig's arguments by describing some additional ways in which the application of the Cheng-Prusoff method to pharmacological experiments are likely to result in misinterpretation and erroneous estimates of antagonist affinity.

引用

页码：110 / 112

页数：3