FLOW CYTOMETRY - HIGH-RESOLUTION INSTRUMENT FOR EVERYONE

被引:134
作者
STEEN, HB
LINDMO, T
机构
[1] Department of Biophysics, Norsk Hydro's Institute for Cancer Research, Montebello
关键词
D O I
10.1126/science.441727
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A new flow configuration for flow cytometry has been devised in which a flat, laminar stream of water, containing the stained cells in a narrow central sector, is formed on a microscope cover slip by a pressurized jet of water directed onto the glass at low angle. The stream of cells is viewed by means of a fluorescence microscope with incident illumination and a pulse photometer. Coupled to a multichannel pulse height analyzer, the instrument constitutes a stable and easy-to-operate flow cytometer with a resolution equal to or better than a coefficient of variance of 1.4 percent in measurements of cellular DNA. Copyright © 1979 AAAS.
引用
收藏
页码:403 / 404
页数:2
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