ALIPHATIC AND AROMATIC INHIBITORS BINDING TO THE ACTIVE-SITE OF CATECHOL 2,3-DIOXYGENASE FROM PSEUDOMONAS-PUTIDA MT-2

被引：25

作者：

BERTINI, I ^{[1
]}

BRIGANTI, F ^{[1
]}

SCOZZAFAVA, A ^{[1
]}

机构：

[1] UNIV FLORENCE, DIPARTIMENTO CHIM, CHIM INORGAN & BIOINORGAN LAB, I-50121 FLORENCE, ITALY

来源：

FEBS LETTERS | 1994年 / 343卷 / 01期

关键词：

CATECHOL; DIOXYGENASE; EXTRADIOL CLEAVAGE; PSEUDOMONAS PUTIDA;

D O I：

10.1016/0014-5793(94)80606-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of different classes of inhibitors with the extradiol cleaving catechol 2,3-dioxygenase from Pseudomonas putida mt-2 was monitored by longitudinal and transverse proton relaxation measurements as well as by kinetic activity studies in order to characterize the type of interaction of such inhibitors with the active site of the enzyme. The average distances of the inhibitors from the catalytic iron(II) ion have been estimated from the H-1 longitudinal relaxation rates. Phenols and aliphatic ketones appear to be coordinated to the iron(II) ion in the active site.

引用

页码：56 / 60

页数：5

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