THE EFFECT OF SUCROSE AND TREHALOSE ON VIABILITY OF ONE-CELL AND 2-CELL RABBIT EMBRYOS

被引:11
作者
SMORAG, Z [1 ]
HEYMAN, Y [1 ]
GARNIER, V [1 ]
GAJDA, B [1 ]
机构
[1] INRA,PHYSIOL ANIM STN,F-78350 JOUY EN JOSAS,FRANCE
关键词
dehydration; one- and two-cell rabbit embryos; sucrose; trehalose;
D O I
10.1016/0093-691X(90)90551-4
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The effect of sucrose and trehalose on the viability of one- and two-cell rabbit embryos was investigated. A significant decrease in the viability of one- and two-cell embryos exposed for 30 min. at 20°C was observed. At 38°C none of the two-cell embryos in a sucrose solution survived after 30 min exposure, while approximately 50% of the embryos survived in a trehalose solution. The cleveage rate in culture of two-cell embryos exposed both to 2.0 M or 1.45 M trehalose was significantly lower in comparison with the control group. However the survival rate after transfer of two-cell embryos exposed to 1.45 M trehalose solution at 20°C remained the same as that of the control group. © 1990.
引用
收藏
页码:741 / 747
页数:7
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