RADIOIMMUNOASSAY FOR ABSCISIC-ACID

被引：47
作者：
WALTON, D
DASHEK, W
GALSON, E
机构：
[1] Department of Environmental and Forest Biology, State University of New York, College of Environmental Science and Forestry, Syracuse, 13210, NY
来源：
PLANTA | 1979年 / 146卷 / 02期
关键词：
Abscisic acid; Radioimmunoassay (ABA);
D O I：
10.1007/BF00388224
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We have developed a radioimmunoassay (RIA) for abscisic acid (ABA) in the 0.1 ng to 2.5 ng range. Antibodies were obtained from rabbits immunized with ABA bound via its carboxyl group to bovine serum albumin. Cross-reactivity studies indicate that ABA esters are completely cross-reactive with ABA, while trans, trans abscisic acid (t-ABA) phaseic acid (PA) and dihydrophaseic acid (DPA) have much lower but significant cross-reactivities. Purification methods which reduce the levels of cross-reacting substances are described. © 1979 Springer-Verlag.
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页码：139 / 145
页数：7
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