A RAPID METHOD FOR COUNTING CELL-NUCLEI USING A PARTICLE SIZER COUNTER

被引：24

作者：

LIN, AA ^{[1
]}

NGUYEN, T ^{[1
]}

MILLER, WM ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,DEPT CHEM ENGN,EVANSTON,IL 60208

来源：

BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 02期

关键词：

D O I：

10.1007/BF00159989

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here an improved method for nuclei counting utilizing Triton-X 100 to reduce the size of cell debris, thereby allowing the use of a particle sizer/counter. Furthermore, nuclei are completely released within 30 seconds, as compared to 1 hour using hypotonic solution. The method is accurate above 0.3 x 10(6) cells/mL.

引用

页码：153 / 156

页数：4