INTEGRATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA INVITRO

被引:220
作者
FARNET, CM
HASELTINE, WA
机构
关键词
AIDS; retrovirus;
D O I
10.1073/pnas.87.11.4164
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.
引用
收藏
页码:4164 / 4168
页数:5
相关论文
共 11 条
[1]   A NUCLEOPROTEIN COMPLEX MEDIATES THE INTEGRATION OF RETROVIRAL DNA [J].
BOWERMAN, B ;
BROWN, PO ;
BISHOP, JM ;
VARMUS, HE .
GENES & DEVELOPMENT, 1989, 3 (04) :469-478
[2]   RETROVIRAL INTEGRATION - STRUCTURE OF THE INITIAL COVALENT PRODUCT AND ITS PRECURSOR, AND A ROLE FOR THE VIRAL IN PROTEIN [J].
BROWN, PO ;
BOWERMAN, B ;
VARMUS, HE ;
BISHOP, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2525-2529
[3]   CORRECT INTEGRATION OF RETROVIRAL DNA INVITRO [J].
BROWN, PO ;
BOWERMAN, B ;
VARMUS, HE ;
BISHOP, JM .
CELL, 1987, 49 (03) :347-356
[4]   RETROVIRAL DNA INTEGRATION - STRUCTURE OF AN INTEGRATION INTERMEDIATE [J].
FUJIWARA, T ;
MIZUUCHI, K .
CELL, 1988, 54 (04) :497-504
[5]  
HIRT B, 1967, J MOL BIOL, V26, P5707
[6]   TEMPORAL ASPECTS OF DNA AND RNA-SYNTHESIS DURING HUMAN IMMUNODEFICIENCY VIRUS-INFECTION - EVIDENCE FOR DIFFERENTIAL GENE-EXPRESSION [J].
KIM, SY ;
BYRN, R ;
GROOPMAN, J ;
BALTIMORE, D .
JOURNAL OF VIROLOGY, 1989, 63 (09) :3708-3713
[7]  
Maniatis T., 1982, MOL CLONING
[8]   ADAPTATION OF THE BICINCHONINIC ACID PROTEIN ASSAY FOR USE WITH MICROTITER PLATES AND SUCROSE GRADIENT FRACTIONS [J].
REDINBAUGH, MG ;
TURLEY, RB .
ANALYTICAL BIOCHEMISTRY, 1986, 153 (02) :267-271
[9]  
VARMUS H, 1989, MOBILE DNA, P53
[10]  
VARMUS HE, 1985, RNA TUMOR VIRUSES, V1