VALIDATION AND CHARACTERIZATION OF THE L5178Y-TK+/- MOUSE LYMPHOMA MUTAGEN ASSAY SYSTEM

被引：488
作者：
CLIVE, D
JOHNSON, KO
SPECTOR, JFS
BATSON, AG
BROWN, MMM
机构：
[1] US EPA,BIOCHEM BRANCH,RES TRIANGLE PK,NC 27711
[2] UNIV N CAROLINA,GENET CURRICULUM,CHAPEL HILL,NC 27514
来源：
MUTATION RESEARCH | 1979年 / 59卷 / 01期
关键词：
D O I：
10.1016/0027-5107(79)90195-7
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The current status of the L5178Y/TK+/-→TK-/- mouse-lymphona mutagenicity assay is described. Dose-survival-mutagenic response data are shown for 43 chemicals. Mutagenicity and cytotoxicity in the presence or absence of non-induced and/or Aroclor-induced rat-liver S-9 are compared for most of these chemicals. 25 of these for which usable carcinogenicity data exist have been used to construct an approximately linear relationship between oncogenic potency in vivo and mutagenic potency in this system in vitro; linearity between these two endpoints extends over a greater than 100 000-fold range in potencies. Several carcinogens which are negative or difficult to detect in the standard Ames assay are mutagenic in this mammalian cell system. These include natulan, sodium saccharin (lot S-1022), p,p′-DDE (a metabolite of DDT), dimethylnitrosamine, diethylnitrosamine and diethylstilbestrol. © 1979.
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页码：61 / 108
页数：48
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