A SIMPLE PROCEDURE FOR PURIFICATION OF YEAST PHENYLALANINE TRANSFER RNA

被引：56

作者：

LITT, M

机构：

[1] Department of Biochemistry University, Oregon Medical School Portland

[2] Division of Experimental Medicine University, Oregon Medical School Portland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1968年 / 32卷 / 03期

关键词：

D O I：

10.1016/0006-291X(68)90691-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For the past several years we have been attempting to study the secondary and tertiary structure of tRNAs+ + Abbreviations used in this paper are the same as those used by RajBhandary, Stuart and Chang (1968) with the following additions: BD-cellulose, benzoylated DEAE cellulose; pmole, 10-12 mole. by means of chemical probes. In the course of this work it became necessary to obtain a highly purified species of tRNA. For two reasons we chose to purify yeast Phenylalanine tRNA. The nucleotide sequence of this molecule has been completely worked out (RajBhandary and Chang [1968]). Secondly, a simple and highly effective partial purification of this species has been obtained by Gillam et al. (1967) on benzoylated DEAE cellulose. In this communication we report the use of BD-cellulose to complete the purification of tRNAPhe. © 1968.

引用

页码：507 / &