ACTIVATION OF RAF-1 BY 14-3-3-PROTEINS

被引：330

作者：

FANTL, WJ ^{[1
]}

MUSLIN, AJ ^{[1
]}

KIKUCHI, A ^{[1
]}

MARTIN, JA ^{[1
]}

MACNICOL, AM ^{[1
]}

GROSS, RW ^{[1
]}

WILLIAMS, LT ^{[1
]}

机构：

[1] WASHINGTON UNIV, SCH MED, DIV BIOORGAN CHEM & MOLEC PHARMACOL, ST LOUIS, MO 63110 USA

来源：

NATURE | 1994年 / 371卷 / 6498期

关键词：

D O I：

10.1038/371612a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

THE protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21(ras) (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood(4-13). Using the yeast two-hybrid systems(8,14-16), we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA(2)) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins(17-23). Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

引用

页码：612 / 614

页数：3

共 30 条

[1] 14-3-3 PROTEINS - A HIGHLY CONSERVED, WIDESPREAD FAMILY OF EUKARYOTIC PROTEINS [J].

AITKEN, A ;

COLLINGE, DB ;

VANHEUSDEN, BPH ;

ISOBE, T ;

ROSEBOOM, PH ;

ROSENFELD, G ;

SOLL, J .

TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (12) :498-501

[2] REGULATION OF THE YEAST HO GENE [J].

BREEDEN, L ;

NASMYTH, K .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1985, 50 :643-650

[3] ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE BY V-RAF IN NIH 3T3 CELLS AND INVITRO [J].

DENT, P ;

HASER, W ;

HAYSTEAD, TAJ ;

VINCENT, LA ;

ROBERTS, TM ;

STURGILL, TW .

SCIENCE, 1992, 257 (5075) :1404-1407

[4]

DU XP, 1994, J BIOL CHEM, V269, P18287

[5] THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT [J].

DURFEE, T ;

BECHERER, K ;

CHEN, PL ;

YEH, SH ;

YANG, YZ ;

KILBURN, AE ;

LEE, WH ;

ELLEDGE, SJ .

GENES & DEVELOPMENT, 1993, 7 (04) :555-569

[6] REQUIREMENT FOR RAF AND MAP KINASE FUNCTION DURING THE MEIOTIC MATURATION OF XENOPUS-OOCYTES [J].

FABIAN, JR ;

MORRISON, DK ;

DAAR, IO .

JOURNAL OF CELL BIOLOGY, 1993, 122 (03) :645-652

[7] A NOVEL GENETIC SYSTEM TO DETECT PROTEIN PROTEIN INTERACTIONS [J].

FIELDS, S ;

SONG, OK .

NATURE, 1989, 340 (6230) :245-246

[8] 14-3-3-PROTEIN HOMOLOGS REQUIRED FOR THE DNA-DAMAGE CHECKPOINT IN FISSION YEAST [J].

FORD, JC ;

ALKHODAIRY, F ;

FOTOU, E ;

SHELDRICK, KS ;

GRIFFITHS, DJF ;

CARR, AM .

SCIENCE, 1994, 265 (5171) :533-535

[9] ISOLATION OF THE RB-RELATED P130 THROUGH ITS INTERACTION WITH CDK2 AND CYCLINS [J].

HANNON, GJ ;

DEMETRICK, D ;

BEACH, D .

GENES & DEVELOPMENT, 1993, 7 (12A) :2378-2391

[10]

HARPER JW, 1993, CELL, V75, P805

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