ULTRASTRUCTURAL-LOCALIZATION OF WHEAT-GERM-AGGLUTININ BINDING-SITES ON THE SPERM SURFACE OF WATER-BUFFALO (BUBALUS-BUBALIS) - A FRACTURE LABEL STUDY

被引：0

作者：

BAINS, HK

PABST, MA

WERNER, G

BAWA, SR

机构：

[1] PANJAB UNIV, DEPT BIOPHYS, CHANDIGARH 160014, INDIA

[2] GRAZ UNIV, DEPT HISTOL & EMBRYOL, A-8010 GRAZ, AUSTRIA

[3] SAAR UNIV, FAC MED, HOMBURG, GERMANY

来源：

JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY | 1993年 / 25卷 / 04期

关键词：

SPERMATOZOA; WGA COLLOIDAL GOLD; FRACTURE LABEL; WATER BUFFALO;

D O I：

暂无

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

In the present study we have examined the plasma membrane surface organization employing fluorescein isothiocyanate linked wheat germ agglutinin (WGA) of the cauda epididymal and ejaculated spermatozoa of water buffalo. Intramembrane particle distribution pattern in the various segments of the spermatozoa has also been observed. WGA-ovomucoid gold has been used to study the distribution of sialoproteins on the sperm surface. With fracture label, WGA receptor sites have been identified on the fractured membrane halves of the sperm plasma membrane overlying the acrosome as well as the middle piece and the principal piece.

引用

页码：465 / 470

页数：6

共 16 条

[1] ULTRASTRUCTURAL-LOCALIZATION OF WHEAT-GERM-AGGLUTININ BINDING-SITES ON THE MEMBRANES OF RAT-LIVER MITOCHONDRIA
FRANCOIS, D
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BRUN, JL
MONGIAT, F
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1980, 73 (02): : 148 - 156
[2] AN ULTRASTRUCTURAL-STUDY OF THE SERTOLI-CELL IN THE WATER-BUFFALO (BUBALUS-BUBALIS)
KUROHMARU, M
YAMASHIRO, S
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BASRUR, PK
ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE C, 1992, 21 (01): : 82 - 90
[3] SURFACE LOCALIZATION OF WHEAT-GERM-AGGLUTININ AND CONCANAVALIN A BINDING-SITES ON NORMAL HUMAN-BLOOD CELLS - AN ULTRASTRUCTURAL HISTOCHEMICAL-STUDY
ZINSMEISTER, VAP
ACKERMAN, GA
ANATOMICAL RECORD, 1982, 202 (01): : 167 - 167
[4] SURFACE LOCALIZATION OF WHEAT-GERM-AGGLUTININ AND CONCANAVALIN-A BINDING-SITES ON NORMAL HUMAN-BLOOD CELLS - AN ULTRASTRUCTURAL HISTOCHEMICAL-STUDY
ZINSMEISTER, VAP
ACKERMAN, GA
TISSUE & CELL, 1983, 15 (05): : 673 - 681
[5] ULTRASTRUCTURAL-LOCALIZATION OF CONCANAVALIN-A AND WHEAT-GERM-AGGLUTININ BINDING-SITES IN ADULT AND EMBRYONIC MOUSE MYOCARDIUM - SOME PROBLEMS OF CONTROLS IN METHODS USING PEROXIDASE AS A CYTOCHEMICAL MARKER
GROS, D
BRUCE, B
CHALLICE, CE
SCHREVEL, J
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (03) : 193 - 200
[6] ULTRASTRUCTURAL-LOCALIZATION OF WHEAT-GERM AGGLUTININ-BINDING SITES IN ESCHERICHIA-COLI WITH WHEAT-GERM AGGLUTININ-GOLD COMPLEXES
MORIOKA, H
TACHIBANA, M
SUGANUMA, A
JOURNAL OF ELECTRON MICROSCOPY, 1987, 36 (1-2): : 36 - 39
[7] THE ROLE OF LYMPHOCYTE SURFACE BINDING-SITES FOR WHEAT-GERM-AGGLUTININ IN THE NEGATIVE REGULATION OF CANCER-PATIENTS
TOGE, T
YAMAGUCHI, Y
SAWAMURA, A
SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY, 1993, 23 (09): : 765 - 770
[8] ENDOCYTOSIS OF WHEAT-GERM-AGGLUTININ BINDING-SITES FROM THE CELL-SURFACE INTO A TUBULAR ENDOSOMAL NETWORK
RAUB, TJ
KOROLY, MJ
ROBERTS, RM
JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 143 (01) : 1 - 12
[9] FREEZE-FRACTURE CYTOCHEMISTRY OF RAT GLOMERULAR CAPILLARY TUFT - DETERMINATION OF WHEAT-GERM-AGGLUTININ BINDING-SITES AND LOCALIZATION OF ANIONIC CHARGES
CHEVALIER, J
APPAY, MD
WANG, XY
BARIETY, J
DASILVA, PP
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1987, 35 (12) : 1389 - 1399
[10] LOCALIZATION OF WHEAT-GERM-AGGLUTININ AND ANTIBODY-BINDING SITES ON THE PLASMA-MEMBRANES OF SEA-URCHIN SPERM HEADS AS REVEALED BY LABEL-FRACTURE AND FRACTURE-FLIP
SHEN, RL
WARD, RD
DASILVA, PP
NISHIOKA, D
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1991, 28 (04) : 410 - 418

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