A NEW HYBRID PROMOTER DIRECTS TRANSCRIPTION AT IDENTICAL START POINTS IN MAMMALIAN-CELLS AND IN-VITRO

被引：13

作者：

DIRKS, W

SCHAPER, F

HAUSER, H

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,GENET EUKARYOTES,D-38124 BRAUNSCHWEIG,GERMANY

[2] DSM,GERMAN COLLECT MICROORGANISMS & CELL CULTURES,DEPT HUMAN & ANIM CELL CULTURE,D-38124 BRAUNSCHWEIG,GERMANY

来源：

GENE | 1994年 / 149卷 / 02期

关键词：

RECOMBINANT DNA; MAMMALIAN EXPRESSION VECTOR; MPSV-LTR; T7; PROMOTER; T3;

D O I：

10.1016/0378-1119(94)90187-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A hybrid promoter which generates large amounts of mRNAs with transcription start points (tsp) differing in one nt, both in mammalian cells and in vitro, was constructed by integrating the promoter of bacteriophage T7 in between the TATA box and the tsp of the retroviral myeloproliferative sarcoma virus (MPSV) long terminal repeat (LTR). This promoter was designed for sequence and secondary structure studies of 5' untranslated regions (UTR) with respect to mRNA stability and translatability.

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页码：389 / 390

页数：2

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