ISOLATION OF A THERMOSTABLE ENZYME CATALYZING DISULFIDE BOND FORMATION FROM THE ARCHAEBACTERIUM SULFOLOBUS-SOLFATARICUS

被引：18
作者：
GUAGLIARDI, A
CERCHIA, L
DEROSA, M
ROSSI, M
BARTOLUCCI, S
机构：
[1] NAPLES UNIV,DIPARTIMENTO CHIM ORGAN & BIOL,VIA MEZZOCANNONE 16,I-80134 NAPLES,ITALY
[2] CNR,IST BIOCHIM PROT & ENZIMOL,I-80125 NAPLES,ITALY
来源：
FEBS LETTERS | 1992年 / 303卷 / 01期
关键词：
ARCHAEBACTERIA; DISULFIDE BOND FORMATION; THERMOSTABLE ENZYME;
D O I：
10.1016/0014-5793(92)80470-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A disulfide bond-forming enzyme was purified from the cytosol of the archaebacterium Sulfolobus solfataricus, strain MT-4. The enzyme, assayed by its ability to oxidize and reactivate reductively denatured ribonuclease A, had a small molecular size and displayed a high thermostability. The N-terminal amino acid sequence is reported.
引用
收藏
页码：27 / 30
页数：4
相关论文
共 15 条
[1]   KINETICS OF FORMATION OF NATIVE RIBONUCLEASE DURING OXIDATION OF REDUCED POLYPEPTIDE CHAIN [J].
ANFINSEN, CB ;
HABER, E ;
SELA, M ;
WHITE, FH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1961, 47 (09) :1309-+
[2]  
BARTOLUCCI S, 1987, J BIOL CHEM, V262, P7725
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   TISSUE SULFHYDRYL GROUPS [J].
ELLMAN, GL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77
[5]  
FREEDMAN RB, 1989, BIOCHEM SOC SYMP, V55, P167
[6]   STABILITY AND ACTIVITY OF A THERMOSTABLE MALIC ENZYME IN DENATURANTS AND WATER-MISCIBLE ORGANIC-SOLVENTS [J].
GUAGLIARDI, A ;
MANCO, G ;
ROSSI, M ;
BARTOLUCCI, S .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 183 (01) :25-30
[7]   PATHWAYS OF FOLDING OF REDUCED BOVINE PANCREATIC RIBONUCLEASE [J].
HANTGAN, RR ;
HAMMES, GG ;
SCHERAGA, HA .
BIOCHEMISTRY, 1974, 13 (17) :3421-3431
[8]  
HOLMGREN A, 1979, J BIOL CHEM, V254, P9627
[9]  
HOLMGREN A, 1989, J BIOL CHEM, V264, P13963
[10]   SULFHYDRYL OXIDASE-CATALYZED FORMATION OF DISULFIDE BONDS IN REDUCED RIBONUCLEASE [J].
JANOLINO, VG ;
SWAISGOOD, HE .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 258 (01) :265-271
12