DETECTION OF ENTERIC VIRUSES IN OYSTERS BY USING THE POLYMERASE CHAIN-REACTION

被引：143

作者：

ATMAR, RL

METCALF, TG

NEILL, FH

ESTES, MK

机构：

[1] BAYLOR COLL MED, DEPT MED, 1 BAYLOR PLAZA, HOUSTON, TX 77030 USA

[2] BAYLOR COLL MED, DIV MOLEC VIROL, HOUSTON, TX 77030 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 02期

关键词：

D O I：

10.1128/AEM.59.2.631-635.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A procedure for the detection of enteric viral nucleic acid in oysters by the polymerase chain reaction was developed. Known quantities of poliovirus type 1 were seeded into oysters. Virus was extracted and concentrated by using organic flocculation and polyethylene glycol precipitation. Inhibitors of reverse transcription-polymerase chain reaction were present in the oyster extracts, preventing amplification of target viral nucleic acid. The use of cetyltrimethylammonium bromide precipitation sufficiently removed inhibitors to allow detection of as few as 10 PFU of poliovirus. Norwalk virus also could be detected after being seeded into oysters. This methodology may be useful for the detection of these and other shellfish-borne viral pathogens.

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页码：631 / 635

页数：5

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