SKELETAL KERATAN SULFATE CHAIN MOLECULAR-WEIGHT CALIBRATION BY HIGH-PERFORMANCE GEL-PERMEATION CHROMATOGRAPHY

被引：16
作者：
DICKENSON, JM
MORRIS, HG
NIEDUSZYNSKI, IA
HUCKERBY, TN
机构：
[1] UNIV LANCASTER,INST ENVIRONM & BIOL SCI,DIV BIOL SCI,LANCASTER LA1 4YQ,ENGLAND
[2] UNIV LANCASTER,DEPT CHEM,LANCASTER LA1 4YA,ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 190卷 / 02期
关键词：
D O I：
10.1016/0003-2697(90)90192-C
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gelpermeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 m NaCl and at 30°C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship log10Mr = 4.588 - 2.128Kav which is applicable in the range Kav = 0.15-0.65. © 1990.
引用
收藏
页码：271 / 275
页数：5
相关论文
共 26 条
[1]  
BRAY BA, 1967, J BIOL CHEM, V242, P3373
[2]  
CARLSON DM, 1968, J BIOL CHEM, V243, P616
[3]  
CATERSON B, 1983, J BIOL CHEM, V258, P8848
[4]   2 LINKAGE-REGION FRAGMENTS ISOLATED FROM SKELETAL KERATAN SULFATE CONTAIN A SULFATED N-ACETYLGLUCOSAMINE RESIDUE [J].
DICKENSON, JM ;
HUCKERBY, TN ;
NIEDUSZYNSKI, IA .
BIOCHEMICAL JOURNAL, 1990, 269 (01) :55-59
[5]  
FUNDERBURGH JL, 1987, J BIOL CHEM, V262, P11634
[6]   MOLECULAR-WEIGHT DISTRIBUTION OF GLYCOSAMINOGLYCANS [J].
HOPWOOD, JJ ;
ROBINSON, HC .
BIOCHEMICAL JOURNAL, 1973, 135 (04) :631-637
[7]   1H-NMR STUDIES AT 500-MHZ OF A NEUTRAL DISACCHARIDE AND SULFATED DI-OLIGOSACCHARIDES, TETRA-OLIGOSACCHARIDES, HEXA-OLIGOSACCHARIDES AND LARGER OLIGOSACCHARIDES OBTAINED BY ENDO-BETA-GALACTOSIDASE TREATMENT OF KERATAN SULFATE [J].
HOUNSELL, EF ;
FEENEY, J ;
SCUDDER, P ;
TANG, PW ;
FEIZI, T .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 157 (02) :375-384
[8]  
HUCKERBY TN, 1990, IN PRESS MAGN RESON
[9]   MOLECULAR-WEIGHT RANGE OF MUCOSAL-HEPARIN PREPARATIONS [J].
JOHNSON, EA ;
MULLOY, B .
CARBOHYDRATE RESEARCH, 1976, 51 (01) :119-127
[10]  
KRUSIUS T, 1986, J BIOL CHEM, V261, P8237
123