PRODUCTION OF HYDROGEN-PEROXIDE BY ARYL-ALCOHOL OXIDASE FROM THE LIGNINOLYTIC FUNGUS PLEUROTUS-ERYNGII

被引：110

作者：

GUILLEN, F ^{[1
]}

MARTINEZ, AT ^{[1
]}

MARTINEZ, MJ ^{[1
]}

机构：

[1] CSIC, CTR INVEST BIOL, UNIDAD MICROBIOL APLICADA, VELAZQUEZ 144, E-28006 MADRID, SPAIN

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1990年 / 32卷 / 04期

关键词：

D O I：

10.1007/BF00903784

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Production of extracellular hydrogen peroxide by fungal oxidases is been investigated as a requirement for lignin degradation. Aryl-alcohol oxidase activity is described in extracellular liquid and mycelium of Pleurotus eryngii and studied under non-limiting nitrogen conditions. This aryl-alcohol oxidase catalyses conversion of primary aromatic alcohols to the corresponding aldehydes and H2O2, showing no activity with aliphatic and secondary aromatic alcohols. The enzyme is stable at pH 4.0-9.0, has maximal activity at 45°-50°C and pH 6.0-6.5, is inhibited by Ag+, Pb2+ and NaN3, and has a Km of 1.2 m M using veratryl alcohol as substrate. A single protein band with aryl-alcohol oxidase activity was found in zymograms of extracellular and intracellular crude enzyme preparations from P. eryngii. © 1990 Springer-Verlag.

引用

页码：465 / 469

页数：5

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