MICROTUBULAR RESPONSE TO COLCHICINE IN ADULT AND FETAL-RAT HEPATOCYTES

被引：8
|
作者：
KAUFMAN, SS
BLAIN, PL
PARK, JHY
TUMA, DJ
机构：
[1] VET ADM MED CTR,LIVER STUDY UNIT,OMAHA,NE 68105
[2] CREIGHTON UNIV,SCH MED,DEPT PEDIAT,OMAHA,NE 68178
[3] UNIV NEBRASKA,MED CTR,COLL MED,DEPT BIOCHEM,OMAHA,NE 68105
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1990年 / 95卷 / 02期
关键词：
D O I：
10.1016/0300-9629(90)90211-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. 1. Colchicine and related anti-microtubular drugs impair plasma protein secretion from adult rat liver expiants 2-3-fold more than from fetal tissue. 2. 2. Indirect immunofluorescence microscopy of cultured adult and fetal hepatocytes demonstrated that hepatocytes of both ages contain large numbers of densely packed microtubules which are equally disassembled by 10 μM colchicine. 3. 3. Colchicine (10 μM) reduced secretion of [14C]leucine-labelled proteins from cultured adult hepatocytes by about 50% but did not significantly impede fetal secretion. 4. 4. These results confirmed that plasma protein secretion can proceed without an intact microtubular system in fetal hepatocytes. © 1990.
引用
收藏
页码：281 / 290
页数：10
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