EVALUATION OF A COMMERCIAL RIBOSOMAL-RNA TARGET AMPLIFICATION ASSAY FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX IN RESPIRATORY SPECIMENS

被引：24
作者：
EHLERS, S
PIRMANN, M
ZAKI, W
HAHN, H
机构：
[1] Institut für Medizinische Mikrobiologie und Infektionsimmunologie, Freie Universität Berlin, Berlin, 12203
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 1994年 / 13卷 / 10期
关键词：
D O I：
10.1007/BF02111344
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Seventy-one respiratory samples were analyzed in a new commercially available target rRNA amplification assay aimed at detecting Mycobacterium tuberculosis complex directly in patient specimens. The assay (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test) correctly identified 26 of 27 samples positive for Mycobacterium tuberculosis and 43 of 44 samples negative for Mycobacterium tuberculosis in culture. When appropriate controls are performed, the assay also performs well with other specimens, such as tissue biopsies, lymph node or bone marrow aspirates and pleural and ascitic exudates.
引用
收藏
页码：827 / 829
页数：3
相关论文
共 16 条
[1]   DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN-REACTION AND GEN-PROBE AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DIRECT TEST [J].
ABE, C ;
HIRANO, K ;
WADA, M ;
KAZUMI, Y ;
TAKAHASHI, M ;
FUKASAWA, Y ;
YOSHIMURA, T ;
MIYAGI, C ;
GOTO, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (12) :3270-3274
[2]   COMPARISON OF MB-CHECK, BACTEC, AND EGG-BASED MEDIA FOR RECOVERY OF MYCOBACTERIA [J].
ABE, C ;
HOSOJIMA, S ;
FUKASAWA, Y ;
KAZUMI, Y ;
TAKAHASHI, M ;
HIRANO, K ;
MORI, T .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (04) :878-881
[3]   POLYMERASE CHAIN-REACTION FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN SPUTUM [J].
ANDERSEN, AB ;
THYBO, S ;
GODFREYFAUSSETT, P ;
STOKER, NG .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1993, 12 (12) :922-927
[4]  
ARNOLD LJ, 1989, CLIN CHEM, V35, P1588
[5]   DIRECT DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY DNA AMPLIFICATION [J].
DEWIT, D ;
STEYN, L ;
SHOEMAKER, S ;
SOGIN, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (11) :2437-2441
[6]   POLYMERASE CHAIN-REACTION AMPLIFICATION OF A REPETITIVE DNA-SEQUENCE SPECIFIC FOR MYCOBACTERIUM-TUBERCULOSIS [J].
EISENACH, KD ;
CAVE, MD ;
BATES, JH ;
CRAWFORD, JT .
JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (05) :977-981
[7]   DETECTION AND IDENTIFICATION OF MYCOBACTERIA BY AMPLIFICATION OF MYCOBACTERIAL DNA [J].
HANCE, AJ ;
GRANDCHAMP, B ;
LEVYFREBAULT, V ;
LECOSSIER, D ;
RAUZIER, J ;
BOCART, D ;
GICQUEL, B .
MOLECULAR MICROBIOLOGY, 1989, 3 (07) :843-849
[8]   SPECIFIC DETECTION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX STRAINS BY POLYMERASE CHAIN-REACTION [J].
HERMANS, PWM ;
SCHUITEMA, ARJ ;
VANSOOLINGEN, D ;
VERSTYNEN, CPHJ ;
BIK, EM ;
THOLE, JER ;
KOLK, AHJ ;
VANEMBDEN, JDA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (06) :1204-1213
[9]   COLLABORATIVE FEASIBILITY STUDY OF A BIPHASIC SYSTEM (ROCHE SEPTI-CHEK AFB) FOR RAPID DETECTION AND ISOLATION OF MYCOBACTERIA [J].
ISENBERG, HD ;
DAMATO, RF ;
HEIFETS, L ;
MURRAY, PR ;
SCARDAMAGLIA, M ;
JACOBS, MC ;
ALPERSTEIN, P ;
NILES, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (08) :1719-1722
[10]   DETECTION AND IDENTIFICATION OF MYCOBACTERIUM-TUBERCULOSIS DIRECTLY FROM SPUTUM SEDIMENTS BY AMPLIFICATION OF RIBOSOMAL-RNA [J].
JONAS, V ;
ALDEN, MJ ;
CURRY, JI ;
KAMISANGO, K ;
KNOTT, CA ;
LANKFORD, R ;
WOLFE, JM ;
MOORE, DF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (09) :2410-2416
12