RAPID METHOD FOR COMPLETE CHEMICAL CHARACTERIZATION OF SIMPLE AND ACYLATED ANTHOCYANINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY GAS-LIQUID-CHROMATOGRAPHY

被引：51

作者：

GAO, L ^{[1
]}

MAZZA, G ^{[1
]}

机构：

[1] AGR CANADA,RES STN,FOOD RES LAB,SUMMERLAND V0H 1ZO,BC,CANADA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1994年 / 42卷 / 01期

关键词：

D O I：

10.1021/jf00037a020

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A rapid method for complete chemical characterization of anthocyanins has been developed. The method uses a single acid hydrolysis of anthocyanins in methanol-2 N HCl to effectively cleave the intact pigments into stable aglycons, sugars, and acyl groups. The anthocyanidins and phenolic acids are characterized by reversed-phase high-performance liquid chromatography (RP-HPLC) in a single analytical run using retention times and UV-vis absorption spectra. The aliphatic acylating acids and the sugars are characterized by capillary gas-liquid chromatography (GLC) analysis of trimethylsilyl derivatives of sugars and aliphatic acids and methyl esters of aliphatic acids. This procedure, which is fast and reliable, requires minimal sample size and should be useful in the characterization of anthocyanins.

引用

页码：118 / 125

页数：8

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