DERIVATIZATION OF DL-AMINO ACIDS BY URETHANE-PROTECTED ALPHA-AMINO-ACID N-CARBOXYANHYDRIDES FOR SEPARATION BY LC

被引：11

作者：

BRUCKNER, H

LUPKE, M

机构：

[1] Institute of Food Technology, University of Hohenheim, Stuttgart

来源：

CHROMATOGRAPHIA | 1995年 / 40卷 / 9-10期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; AMINO ACID ANALYSIS; CHIRALITY; DIASTEREOISOMERS; DIPEPTIDES;

D O I：

10.1007/BF02290275

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a robotic autosampler, free DL-amino acids were derivatized with highly reactive, urethane-protected, L-alpha-amino acid N-carboxyanhydrides (NCAs) with structures: Boc-Phe-NCA, Boc-Asn(Trt)-NCA, Fmoc-Lys(Boc)-NCA, Fmoc-Met-NCA, Fmoc-Ala-NCA, Z-Ala-NCA, Z-Val-NCA and Z-Leu-NCA (Boc = tert-butyloxycarbonyl, Trt = trityl, Fmoc = 9-(fluor-enyl-methyl)oxycarbonyl, Z = benzyloxycarbonyl). Using sodium berate buffer and acetonitrile solvent, derivatization was complete in 3.5 min at room temperature. By selection of an appropriate reagent, the resulting diastereomeric, N-protected dipeptides were separated on an octylsilica stationary phase using mixtures of sodium acetate buffer and acetonitrile as eluents.

引用

页码：601 / 606

页数：6

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