THE COMPLETE AMINO-ACID-SEQUENCE OF RIBONUCLEASE FROM THE SEEDS OF BITTER GOURD (MOMORDICA-CHARANTIA)

被引：77

作者：

IDE, H ^{[1
]}

KIMURA, M ^{[1
]}

ARAI, M ^{[1
]}

FUNATSU, G ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC AGR,PROT CHEM & ENGN LAB,FUKUOKA 812,JAPAN

来源：

FEBS LETTERS | 1991年 / 284卷 / 02期

关键词：

RIBONUCLEASE; MOMORDICA-CHARANTIA; AMINO ACID SEQUENCE; SEQUENCE COMPARISON;

D O I：

10.1016/0014-5793(91)80675-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of ribonuclease (RNase MC) from the seeds of bitter gourd (Momordica charantia) has been determined. This has been achieved by the sequence analysis of peptides derived by enzymatic digestion with trypsin, lysylendopeptidase, and chymotrypsin, as well as by chemical cleavage with cyanogen bromide. The protein contains 191 amino acid residues and has a calculated molecular mass of 21259 Da. Comparison of this sequence with sequences of the fungal RNases, RNase T2, and RNase Rh, revealed that there are highly conserved residues at positions 32-38 (TXHGLWP) and 81-92 (FWXHEWXKHGTC). Furthermore, the sequence of RNase MC was found to be homologous to those of Nicotiana alata S-glycoproteins involved in self-incompatibility sharing 41% identical residues.

引用

页码：161 / 164

页数：4

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