PURIFICATION OF AN ENTEROTOXIN PRODUCED BY BACILLUS-CEREUS BY IMMUNOAFFINITY CHROMATOGRAPHY USING A MONOCLONAL-ANTIBODY

被引：9
|
作者：
SHINAGAWA, K [1 ]
TAKECHI, T [1 ]
MATSUSAKA, N [1 ]
SUGII, S [1 ]
机构：
[1] KITASATO UNIV,SCH MED TECHNOL,DEPT SEROL & IMMUNOL,SAGAMIHARA,KANAGAWA 228,JAPAN
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 1992年 / 38卷 / 02期
关键词：
PURIFICATION; IMMUNOAFFINITY CHROMATOGRAPHY; ENTEROTOXIN; MONOCLONAL ANTIBODY; BACILLUS-CEREUS;
D O I：
10.1139/m92-025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A murine monoclonal antibody (MAb) with high reactivity to an enterotoxin produced by Bacillus cereus was used to prepare an immunoadsorbent for purification of the enterotoxin. By immunoaffinity chromatography using the immunoadsorbent, approximately 25% of crude enterotoxin applied was recovered in the eluate. The purified enterotoxin was found to be electrophoretically and antigenically homogeneous. It also showed vascular permeability activity and mouse lethality, and caused fluid accumulation in mouse ligated intestinal loops, whereas it did not show any hemolytic and lecithinase activities. Thus, immunoaffinity chromatography proved useful in the purification of enterotoxin produced by B. cereus in terms of recovery, purity, and relative ease of performing the purification.
引用
收藏
页码：153 / 156
页数：4
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