NONSPECIFIC STAINING - ITS CONTROL IN IMMUNOFLUORESCENCE EXAMINATION OF SOIL

被引：125

作者：

BOHLOOL, BB

SCHMIDT, EL

机构：

[1] Department of Soil Science, University of Minnesota, St. Paul, MN

[2] Departments of Microbiology and Soil Science, University of Minnesota, Minneapolis, MN

来源：

SCIENCE | 1968年 / 162卷 / 3857期

关键词：

D O I：

10.1126/science.162.3857.1012

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counter-stain as well as to prevent nonspecific staining.

引用

页码：1012 / &