POLYMERIC LANGMUIR FILMS WITH GLUCOSE-OXIDASE AS PROTOTYPE BIOSENSORS

Adsorbed layers of glucose oxidase on monolayers of specially synthesized N, N-dimethyl,N-cetylacetyl,methacryloylethyl-ammonium bromide have been prepared and investigated. The enzyme is strongly adsorbed on a positively charged lipid monolayer and retains its enzymatic activity. The polymerization of the lipid layer by soft UV illumination at the interface increases the stability of the lipid-protein film and the reproducibility of the response measurements. A prototype biosensor is obtained by Langmuir-Blodgett transfer of one to ten lipid-protein monolayers onto Pt electrodes (at 20-40 mN m(-1)) with a measurement range of 0.4-4.0 mM glucose.