HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION AND DIODE-ARRAY UV CONFIRMATION OF ISONICOTINIC-ACID HYDRAZIDE IN CATTLE MILK

被引：14
|
作者：
DEFILIPPI, A [1 ]
PIANCONE, G [1 ]
LAIA, RC [1 ]
BALLA, S [1 ]
TIBALDI, GP [1 ]
机构：
[1] USSL 40,SANITA PUBBL LAB,I-10015 IVREA,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 02期
关键词：
D O I：
10.1016/0378-4347(94)00131-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the determination of isonicotinic acid hydrazide (isoniazid) in milk was developed. Milk was deproteinized with trichloroacetic acid. Isoniazid was condensed with cinnamaldehyde and assayed on a reversed-phase HPLC system, with good sensitivity and accuracy (10 mu g/l) with UV detection at 330 nm. Use of solid-phase extraction with a C-18 cartridge allows the detection limit to be lowered to 0.1 mu g/l with UV detection and confirmation of isoniazid hydrazone from the diode-array UV spectrum.
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页码：466 / 471
页数：6
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