IMMUNOPRECIPITATION OF ATP-SYNTHETASE FROM BEEF-HEART MITOCHONDRIA

被引：15
作者：
LUDWIG, B
CAPALDI, RA
机构：
[1] Institute of Molecular Biology University of Oregon, Eugene
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 04期
关键词：
D O I：
10.1016/S0006-291X(79)80029-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Antibody against chloroform-treated beef heart mitochondrial F1ATPase has been raised in rabbits. The purified antibody inhibits ATPase activity in soluble and membrane bound F1ATPase. It immunoprecipitates the entire ATP synthetase complex (F1ATPase plus membrane sector) from mitochondria. The polypeptide profile of the immunoprecipitate is similar to that of oligomycin-sensitive ATPase purified according to Berden and Voorn-Brouwer (Biochim. Biophys. Acta 501, 424-439, 1978). © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.
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页码：1159 / 1167
页数：9
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